![]() Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.) Filing date Publication date Application filed by Chadeloid Corp filed Critical Chadeloid Corp Priority to US234475A priority Critical patent/US2691596A/en Priority claimed from US435911A external-priority patent/US2802746A/en Application granted granted Critical Publication of US2691596A publication Critical patent/US2691596A/en Anticipated expiration legal-status Critical Status Expired - Lifetime legal-status Critical Current Links Original Assignee Chadeloid Corp Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.) Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.) Expired - Lifetime Application number US234475A Inventor Herman R Nack Waldie William Allshire Current Assignee (The listed assignees may be inaccurate. Google Patents Stain and method of its preparationĭownload PDF Info Publication number US2691596A US2691596A US234475A US23447551A US2691596A US 2691596 A US2691596 A US 2691596A US 234475 A US234475 A US 234475A US 23447551 A US23447551 A US 23447551A US 2691596 A US2691596 A US 2691596A Authority US United States Prior art keywords water stain precipitate weight salt Prior art date Legal status (The legal status is an assumption and is not a legal conclusion. Google Patents US2691596A - Stain and method of its preparation They may be used as an easily observable indicator of stain intensity when NM is not present in a test section.US2691596A - Stain and method of its preparation The Purkinje cells of the cerebellum tend to exhibit the same thionin staining characteristics as the neurons of N. Contaminating the thionin with acetic acid-alcohol from destained slides will alter stain specificity. Prolonged exposure to acetic acid-alcohol will prevent restaining. Then rinse them very well to remove the acid before restaining for a shorter time, or in the dilute thionin. ![]() Dip slides into the acetic acid-alcohol until the color disappears - usually a matter of a few seconds. Overstaining may be overcome by returning slides to a dish filled with 95% ethanol containing a few drops of glacial acetic acid. Residual thionin in the clearing alcohols may impart a background coloration that interferes with other people's slides. Dye-laden water and alcohol will interfere with the destaining. Remember to change your destaining solutions when you are through, be considerate of others. The initial coloration will be purple, but the alcohols will "blue" the stained sections. The staining action is influenced by choice of fixative, fixation time, embedment, section thickness, or section treatments. Inspect this test slide to determine if time in the stain solution should be changed. This results in precipitate particles on your sections and a poorly soluble sludge in the bottom of the staining dish.Īlways stain one slide as a test before committing an entire rack of slides to thionin stain. ![]() Phosphate buffers, including PBS, will cause thionin to precipitate. Slides must be rinsed or rehydrated in distilled water before placing into thionin. Staining times vary from 30 seconds to 30 minutes with this stain. The weak solution is recommended for autoradiography slides, where the emulsion tends to encourage over-staining. ![]() Staining time is usually from 30 seconds to 20 minutes.ī. This is the stain for routine Nissl stains. Mix buffer reagents together and adjust pH before mixing with the thionin stock in the formulas below.Ī.ğull strength thionin stain (1%), 400 ml, pH 4.0. Use high purity thionin, such as Sigma T3387. Filter the solution after the dye has dissolved. Replenish by adding freshly filtered thionin working solution to replace about 10% of the solution in the staining dish (for example in a dish of 400 ml, replace about 40 ml). Working solutions of thionin should be filtered periodically and replenished when prolonged staining times (over 30 minutes) fail to produce acceptable stain intensity. Thionin binds to acidic proteins and nucleic acids with a specificity determined by the pH of the final staining solution. This stain is specific for DNA and Nissl substance, which is primarily ribosomal RNA.
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